Fig. 1.

Irradiation and LPS stimulation induces TLR4 and miR-146a-5p expression in LX2 cells. a and b qRT-PCR analysis showed the expression of TLR4 in LX2 cells at 24 h a or 48 h b after irradiation and LPS stimulation under various conditions, as indicated. c–f LX2 cells were treated with 8 Gy X-ray irradiation and 50 ng/ml LPS for 24 or 48 h and then qRT-PCR was performed to detect the expression levels of IRAK1 c, TRAF6 d, Bcl-2 e and α-SMA f. g miR-146a-5p expression was examined by qRT-PCR at 24 h after irradiation and LPS stimulation under various conditions, as indicated. h miR-146a-5p expression was assessed by qRT-PCR after treatment with 8 Gy X ray irradiation and 50 ng/ml LPS for the indicated time periods. All data are presented as the mean ± S.E.M. of three independent experiments. *P < 0.05 vs. NRT group; ^#P < 0.05 vs. RT group. NRT non-irradiation treatment, RT irradiation treatment