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. 2018 Feb 19;9(3):285. doi: 10.1038/s41419-017-0171-8

Fig. 1. Effects of elevated intracellular cAMP on ONH astrocytes and RGCs.

Fig. 1

a Immunocytochemial analysis of GFAP and NCAM immunoreactivity in purified ONH astrocytes. Scale bar, 20 μm. b Intracellular cAMP level ONH astrocytes treated with forskolin (10 μM) for 1 h. c PKA activity in ONH astrocytes treated with forskolin (10 μM) for 1 h. d Cell viability analysis using MTT assay in ONH astrocytes treated with forskolin (0–40 μM) for 1 h. e Thy-1 (green)-positive primary RGCs cultures. Scale bar, 20 μm. f Cell viability assay using MTT in cultured RGCs treated with forskolin (10 μM), rolipram (2 μM), or H89 (10 μM) for 48 h. For each determination, the cAMP level was normalized to the protein contents, and the PKA activity and cell viability in controls was normalized to a value of 1.0. Data are shown as the mean ± S.D. (n = 3). **P < 0.01; ***P < 0.001 (two-tailed unpaired Student’s t-test)