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. 2018 Feb 19;9(3):285. doi: 10.1038/s41419-017-0171-8

Fig. 4. Axon degeneration and structural impairment of astrocyte processes in the glial lamina of glaucomatous DBA/2 J mice.

Fig. 4

a Representative SBEM micrographs showed normal healthy morphology of astrocytes and axons in the glial lamina of aged matched non-glaucomatous DBA/2J-Gpnmb+mice. b Representative SBEM micrographs showed abnormal morphology of astrocytes and degenerative axons in the glial lamina of 10-month-old glaucomatous DBA/2 J mice that have moderate damage. c Representative SBEM micrographs showed degenerative astrocytes and severe loss of axons in the glial lamina of glaucomatous DBA/2 J mice that has severe damage. Scale bar, 20 nm. d and e Axonal segmentation from SBEM volumes. Representative 3D reconstruction micrographs showed normal healthy morphology of axons with various colors in the glial lamina of non-glaucomatous DBA/2J-Gpnmb+mice d. Representative 3D reconstruction micrographs showed abnormal morphology of axons with various colors in the glial lamina of glaucomatous DBA/2 J mice that have moderate damage e. f–h Astrocyte process segmentation from SBEM volumes. Representative 3D reconstruction micrographs showed normal healthy morphology of astrocyte processes with various colors in the glial lamina of non-glaucomatous DBA/2J-Gpnmb+mice f. Note the arrangement of astrocyte processes, which is likely to show a shape of hook and loop. Representative 3D reconstruction micrographs showed a loosen arrangement of astrocyte processes with various colors in the glial lamina of glaucomatous DBA/2 J mice that have moderate damage g. Representative 3D reconstruction micrographs showed loss of astrocyte processes with various colors in the glial lamina of glaucomatous DBA/2 J mice that have severe damage h. Scale bar, 20 μm