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. 2018 Feb 20;9(3):300. doi: 10.1038/s41419-018-0373-8

Fig. 3. Gasdermin D-induced whole cell currents are inhibited by blockers of TMEM16F.

Fig. 3

a Whole cell currents obtained in non-stimulated HEK293 cells expressing the N-terminal pore-forming domain of gasdermin D (GD-N). The enhanced whole cell currents detected in these cells were significantly inhibited by CaCCinhAO1 (AO1; 20 µM) or tannic acid (TA; 10 µM). b Corresponding current/voltage relationships for the experiments shown in a. c whole cell currents measured in mock transfected HEK293 cells and cells expressing GD-N in the presence of scrambled RNA (scrbld) or after siRNA-knockdown of TMEM16F. d Corresponding current/voltage relationships. Cells were voltage clamped ± 100 mV (1 s) in steps of 20 mV. Mean±SEM (number of experiments). *Significant inhibition by AO1 and TA, respectively (p < 0.05; paired t-test)