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. 2018 Jan 19;9(2):45. doi: 10.1038/s41419-017-0064-x

Fig. 5. The dual PI3K/mTOR inhibitor BEZ235 rescues the cell phenotype induced by PIK3CA-activating mutations.

Fig. 5

a EC were serum starved and then stimulated with VEGF-A in presence or absence of the indicated inhibitors (BEZ235, Everolimus, MK2206); corresponding lysates were then separated by SDS-PAGE and analyzed with the indicated antibodies. b DNA replication rate was measured by Click-iT EdU Alexa Fluor 647 Imaging kit after treatment of EC with the indicated inhibitors. The percentage of EdU-positive nuclei is indicated in the graph. Data were plotted as the mean from three independent experiments; *P < 0.001 and §P < 0.05, vs. the same VEGF-stimulated and untreated cells. c EC treated with the indicated inhibitors were analyzed by flow cytometer. The percentage of cells with linear Forward Scatter > 150 (large cells) is indicated in the graph. Data were plotted as the mean from three independent experiments; *P < 0.01 and §P < 0.05, vs. the same VEGF-stimulated and untreated cells