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. 2018 Jan 19;9(2):60. doi: 10.1038/s41419-017-0091-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Rat primary midbrain neuron–glia or microglia-deleted cultures were stained with antibody against Iba-1 or TH after 2 days of HD treatment and the representative images were shown. b The density of Iba-1 staining was quantified. c The number of TH⁺ neurons was counted in both neuron–glia and microglia-deleted cultures. Results were expressed as a percentage of controls from three experiments performed in duplicate.*p < 0.05, **p < 0.01; Scale bar = 50 μm