Fig. 6. The integrin αMβ2 is essential for NOX2 activation in response to HD.
a BV2 microglial cells were pre-treated with RGD or anti-αM antibody and then HD-induced superoxide production was measured. c-IgG: control IgG. b The density of red fluorescence of DHE oxidation was quantified. c The effects of RGD or anti-αM antibody on HD-induced membrane translocation of p47phox was detected using Western blot and the density of blots was quantified. d, e HD-induced superoxide production was measured in primary mixed-glia cultures prepared from wild type and αM KO mice and the density of red fluorescence of DHE oxidation was quantified. Results were expressed as a percentage of controls from three experiments performed in duplicate. *p < 0.05, **p < 0.01; n = 3–6; Scale bar = 100 μm