Fig. 7. The activation of Src-Erk signals contributes to HD-induced NOX2 activation in vitro and in vivo.
a BV2 microglia were pre-treated with saracatinib (Src inhibitor) and U0126 (Erk inhibitor) prior to HD and then the production of superoxide was measured using DHE. b The density of red fluorescence of DHE oxidation was quantified. c The effects of saracatinib and U0126 on HD-induced p47phox membrane translocation was detected using Western blot and the density of blots was quantified. Results were expressed as a percentage of controls from three experiments performed in duplicate. d The levels of αM, phosphorylated and total Src and Erk were determined in the midbrain tissues of HD-treated rats by Western blot and the representative blots were shown. e–g The density of blots was quantified. *p < 0.05, **p < 0.01; n = 3–6; Scale bar = 100 μm