Fig. 8. Pharmacological inhibition of αMβ2-NOX2 axis attenuates HD-induced microglial activation and dopaminergic neurodegeneration.
a Rat midbrain neuron–glia cultures were pretreated with anti-αM antibody or apocynin (APO) and then stimulated by HD. Microglial cells were stained with antibody against Iba-1 and the representative images were shown. c-IgG: control IgG. b The density of Iba-1 immunostaining was quantified. c Dopaminergic neurons were stained with antibody against TH and the representative images were shown. d The effects of anti-αM antibody or apocynin on HD-induced dopaminergic neurodegeneration were assessed by THir neuron counts. Results were expressed as a percentage of controls from three experiments performed in duplicate. *p < 0.05, **p < 0.01; Scale bar = 50 μm