Fig. 2. Effects of G9A on wound healing, migration, and invasion ability in GC cells (200×).

a G9A expression in six GC cell lines and one immortalized gastric epithelial cell line, as detected by western blotting. b BGC-823 and MKN-28 cells transfected with sh-G9A lentivirus and G9A overexpression lentivirus, respectively, were subjected to western blotting. c Representative images from BGC-823/sh-G9A, MKN-28/G9A cells, and their control groups were recorded 0 and 48 h after scratching of the cell surface. d The relative distances between the wound edges of GC cells at 0 and 48 h. e The migration and invasive behavior were evaluated by using a Transwell chamber with or without Matrigel after knockdown or overexpression of G9A in BGC-823 or MKN-28 cells. f Histograms showed the average number of migrating cells in multiple fields. g Histograms showed the average number of invading cells in multiple fields. Each value is the mean ± SD of three experiments. **P < 0.01