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. 2018 Feb 15;9(3):278. doi: 10.1038/s41419-018-0322-6

Fig. 5. Reg IV promotes G9A expression via p-ERK/p-SP1 signaling.

Fig. 5

a The G9A and SP1 protein levels in BGC-823 cells treated with SP1 siRNA were measured by western blotting. b G9A and p-SP1 levels in MKN-28 cells transfected with Reg IV. c G9A and p-SP1 changes at the protein level in MKN-28 cells transfected with SP1 siRNA, Reg IV siRNA, or both SP1 and Reg IV siRNA. d ChIP assay for the binding of SP1 to the G9A promoter in BGC-823 cells. e G9A promoter activity changes in MKN-28 cells transfected with SP1 siRNA, Reg IV siRNA, or both SP1 and Reg IV siRNA. f G9A and p-SP1 changes at the protein level in MKN-28 cells treated with PD98059, Reg IV, or both PD98059 and Reg IV. g Histograms showing the numbers of invading GC cells transfected with Reg IV overexpression lentivirus, G9A interference lentivirus, or both Reg IV overexpression lentivirus and G9A interference lentivirus. h Adhesion of GC cells transfected with Reg IV overexpression lentivirus, G9A interference lentivirus, or both Reg IV overexpression lentivirus and G9A interference lentivirus to Matrigel-coated surfaces. i, j Representative images (i) and the number of GC cells (j) adherent to murine peritoneum after cells were transfected with Reg IV overexpression lentivirus, G9A interference lentivirus, or both Reg IV overexpression lentivirus and G9A interference lentivirus. Each value is the mean ± SD of three experiments. *P < 0.05, **P < 0.01