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. 2018 Feb 7;9(2):187. doi: 10.1038/s41419-017-0234-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Venn diagram showing the overlap between strong hypermethylated CpGs (33.3% higher than mESCs) in C-iPSCs and 4F-iPSCs. b Volcano plot showing the differences in methylation level (4F-iPSCs–C-iPSCs) of the common strong hypermethylated CpGs in C-iPSCs and 4F-iPSCs (the overlapped ones in a). P values are calculated by Fisher’s exact test. The black horizontal line indicates p value = 0.05. c Pie chart representing the strong hypermethylated CpGs in 4F-iPSCs compared to C-iPSCs (increased ≥33.3%) with respect to their genomic locations