Fig. 5. Effects of the miR-135b–BMAL1 axis on PC cell proliferation, migration and invasion.

a, b The proliferation rates of MIA PaCa-2 and Panc-1 cells stably transfected with the indicated vectors were assessed by CCK-8 assays. The absorbance was measured at 450 nm. c–f In vitro wound-healing assays and transwell assays of MIA PaCa-2 cells (c, e) and Panc-1 cells (d, f) were performed for evaluating cell migration and invasion. Relative percent of wound closure and the number of cells invaded through the transwell membrane coated with matrigel were quantified after incubation for 24 h. Magnification ×200. Data are representative of at least three similar experiments. *P < 0.05