Fig. 2. NAGLU silencing in H9C2 cardiomyoblasts promotes cellular hypertrophy.
a Phalloidin staining of H9C2 sh-CTR and H9C2 sh-NAGLU actin cytoskeleton. Nuclei were counterstained with DAPI. Scale bar: 10 μm. Cell size enlargement was observed in NAGLU-depleted H9C2 compared to control H9C2. Quantification of cell size is the mean ± SD of three independent experiments. *P < 0.05. b Expression levels of mRNAs coding for ANP and BNP hypertrophic mediators in H9C2 sh-CTR and H9C2 sh-NAGLU as measured by quantitative RT-PCR analysis. The amount of ANP and BNP mRNA was normalized with respect to the amount of 18S ribosomal RNA housekeeping gene. The data reported are the mean ± SD of three independent experiments. *P < 0.05