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. 2018 Feb 21;9(3):302. doi: 10.1038/s41419-018-0370-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunofluorescence assay of Trim59 and F-actin in F1 ESCs transfected with Trim59 siRNA (si-TRIM59) or siRNA control (si-con). b Confocal fluorescence images of embryos at E3.5 and E4.5 stained with Alexa488. Images were acquired using a Bio-Rad Radiance 2100 confocal microscope with a Zeiss 63× oil immersion objective. Scale bar, 10 µm; arrows indicate F-actin polymerization. *P < 0.05, **P < 0.01, ***P < 0.005; Ns no significance