Fig. 8. Silencing CaM impaired the invasion of GBM cells in vivo and prolonged the survival of GBM mice (CaM knockdown group: n = 8, control group: n = 8).

a Representative images of H&E staining of tumor border from mice 3 weeks after tumor implantation (black triangle: disseminated tumor). b Immunohistochemistry analysis of the expression of CaM, MMP2, MT1-MMP and vimentin in CaM-shRNA-treated tumors compared to tumors in the control group (Representative images were presented. Images were taken from three different microscopic visions from every independent experiment). c Mouse weights were monitored in each group after tumor implantation. d Mouse survival in different groups was quantified by a Kaplan–Meier curve