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. 2018 Feb 5;9(2):154. doi: 10.1038/s41419-017-0222-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Cytokine array for MSC-CM from three donors. Each cytokine was detected in duplicate. In each array, blots in the upper-left, upper-right and bottom-left represent positive controls. Target cytokines are indicated using square frames with different colours. Their names are listed in b. b Grey density analyses indicating the levels of cytokines in MSC-CM using Image Pro Plus 6.0 software. The mean value (M) of the grey density of three positive controls was calculated, and the grey density for each point of a target cytokine was divided by M, and this ratio was calculated in duplicate. The ratios were used to compare differences in the cytokine levels among MSC-CM from Donor #1, Donor #2 and Donor #3. Two-way ANOVA analysis was performed. **P ≤ 0.001: significant increase versus other two groups; ^&& ≤ 0.001: significant decrease versus the other two groups. c Human EGF levels in MSC-CM. Each time point consisted of five independent measurements (n = 5). Data represent the mean ± S.D. **P ≤ 0.001: significant increase (2.5 ml of DMEM group versus other two groups, one-way ANOVA). d Human CXCL12 levels in MSC-CM. Each time point consisted of five independent measurements (n = 5). Data represent the mean ± S.D. **P ≤ 0.001: significant increase (2.5 ml of DMEM group versus other two groups, one-way ANOVA)