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. 2018 Jan 26;9(2):130. doi: 10.1038/s41419-017-0131-3

Fig. 1. miR-874 downregulated DOR expression by directly targeting its 3′-UTR.

Fig. 1

a Expression of miR-874 and the DOR in HCC cell lines were detected by qRT-PCR in six cell lines. miR-874 and DOR expression were normalized to U6 and β-actin, respectively. b, c The protein and mRNA levels of the DOR decreased after transfection with the miR-874 mimic, as demonstrated by Western blot and qRT-PCR. d, e The protein and mRNA level of the DOR increased after transfection with the miR-874 inhibitor, as demonstrated by Western blot and qRT-PCR. f The predicted sites of miR-874 binding to the 3′-UTR of the DOR were detected using bioinformatics prediction tools. The mutated site in the 3′-UTR of the DOR is shown. g The effect of miR-874 on luciferase activity induced by the pMIR-DOR-wt, pMIR-DOR-mut-1, and pMIR-DOR-mut-2 reporter plasmids in SK-hep-1 cells was measured via luciferase reporter gene assays. Data are shown as the mean ± SD of three replicates (*p < 0.05)