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. 2018 Jan 26;9(2):130. doi: 10.1038/s41419-017-0131-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Levels of the DOR, phosphorylated PKC, and phosphorylated ERK were detected using Western blot analysis. miR-874-overexpressed cells were treated with 20 ng/ml of EGF or combined with 10 µM of U1026. b, c Ratios of density units for each phospho- to total-protein are shown. Phosphorylation of the EGFR and ERK were downregulated by miR-874 overexpression, and ectopic DOR expression eliminated the promoting effect of miR-874 overexpression on phosphorylation level. The EGF significantly restored inhibition of phosphorylation of the EGFR and ERK induced by miR-101 overexpression. When miR-874-overexpressed SK-hep-1 cells were treated with EGF combined with U1026, EGF-induced phosphorylation of ERK was blocked without interfering with EGFR phosphorylation. d The proliferation-promoting effect of the EGF on miR-874-overexpressing SK-hep-1 cells was blocked by U1026, as demonstrated by an MTT assay. e The invasion-promoting effect of the EGF was blocked by U1026, as demonstrated by transwell assay. f The migration-promoting effect of the EGF was blocked by U1026, as demonstrated by wound-healing assay. Data are shown as the mean ± SD of three replicates (*p < 0.05)