Fig. 3. IGF2 promotes the proliferation of wild-type hepatocytes in culture.

a Microscopic images showed the density and morphology for the cultured hepatocytes with or without IGF2 stimuli at 48 h. b Primary hepatocytes were BrdU-positive after treatment with IGF2 for 48 h. c Quantification of BrdU-positive hepatocytes cultured by 24, 48, and 72 h after stimulation with IGF2 (20, 100, 200 ng/ml). d Ki67-positive primary hepatocytes were found at 48 h after IGF2 treatment. e Quantification of Ki67-positive hepatocytes. *p < 0.05, **p < 0.01 vs. cultured hepatocytes without IGF2 stimuli. Shows are mean ± S.D. Scale bar, 100 µm