Fig. 6. Metformin reduces splenomegaly in Jak2^V617F knockin-induced MPN mice.

a Experimental design for induction of MPN phenotype in mice. Bone marrow cells from Jak2^V617F mice were transplanted into lethally irradiated Pep boy mice. After chimerism evaluation at 4 weeks, mice were randomized and daily treated with vehicle (n = 4) or metformin (125 mg/kg) (n = 5) for 6 weeks. b Spleen images and c weight of vehicle and metformin-treated MPN mice. Scale Bar: 10 mm.*p < 0.05, Mann–Whitney test. d Representative histopathology H&E sections of spleen from vehicle and metformin-treated mice. Magnification of 40× (upper panel) and 100× (lower panel). e Illustrative dot plots of erythroid progenitor analysis in the spleen. f Dispersion graphs showing the percentage of early erythroid progenitors (CD71⁺/Ter119⁺ cells) in spleen and bone marrow. g Dispersion graphs showing the hemoglobin and h hematocrit levels