Fig. 2. Notch1 expression was associated with stemness.

a, d CD133+ U87, LN229, and U251 human glioma cells that formed neurospheres exhibited higher Notch1 activity and higher expression levels of stem cell markers. The scale bar corresponds to 20 µm. b H&E staining and immunohistochemical staining of CD31, Notch1, Nestin, Jagged-1, and CD133 in GBM tissue. The scale bar corresponds to 100 µm. c Hes1 was expressed in tumor cells adjacent to CD31-expressing endothelial cells. Co-expression of Notch1 and Nestin, Notch1, and CD133 showing the corresponding colocalization in the same cells within the GBM tissue. The scale bar corresponds to 50 µm