Fig. 3. Feedback activation of TKI resistance-related signaling in EGFR-TKI-resistant NSCLC cells.

a p-AKT, AKT, nuclear p65, nuclear β-Catenin, nuclear Gli1, and nuclear YAP expression levels were measured in EGFR-TKI-resistant and parental NSCLC cell lines. β-actin was used as a loading control. b p-AKT, AKT, nuclear β-Catenin, and nuclear YAP expression levels were detected in EGFR-TKI-resistant cells treated with or without UNC0638. β-actin was used as a loading control. c Bcl-2 and VEGF mRNA expression levels were assessed by real-time RT-PCR in EGFR-TKI-resistant cells treated with or without UNC0638. d PTEN, TBK1, TRAF6, TRAF4, and PP2A expression levels were measured in EGFR-TKI-resistant and parental NSCLC cells. β-actin was used as a loading control