Fig. 1. JQ1 inhibits the proliferation, migration, and invasion of gastric cancer cell lines.
a Box-plots of BRD4 mRNA levels in normal tissue (NT, patient number = 35) vs primary of tumor (TP, patient number = 415) acquired from TCGA Firebrowse portal are visualized using GraphPad Prism, and the p-value is computed and displayed. b Left: Representative of immunohistochemical (IHC) staining of BRD4 in human NT vs TP tissues. Boxed regions are enlarged to the bottom of each image. Right: IHC staining score summary from 52 gastric cancer samples and the paired normal tissue samples is shown in the table on the right. The Pearson’s chi-square test (χ2 = 68.250, p < 0.001) is utilized to evaluate the likelihood of the different expression levels of BRD4 in NT vs TP samples. c Various gastric cancer cells were treated with JQ1 of indicated concentration for 72 h, and cell proliferation was measured by A490 nm using the CellTiter 96RAQueous One Solution cell proliferation assay (MTS) (Promega). Data represent the mean of three independent experiments. Dot line represents the 50% of growth inhibition. d MKN28 or SGC-7901 cells were treated with DMSO or 5 μM of JQ1 for 24 h or 6 days, and cell proliferation was measured at different time points as in c. e MKN28 cells were seeded in soft-agar and cultured for 15 days with DMSO or 5 μM of JQ1. Representative photographs were taken at day 21. f MKN28 cells were treated with DMSO or 5 μM of JQ1, and cell invasion assay was performed using Transwell invasion chambers (Becton, Dickinson, and Company) according to manufacturer’s instructions. g The wound-healing migration assays for MKN28 cells in the presence of DMSO or 5 μM of JQ1. Representative photographs were taken at 0, 18, and 36 h (left). The percentage of the average speed of wound closure from three independent experiments ± SD is shown on the right