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. 2018 Feb 2;9(2):146. doi: 10.1038/s41419-017-0177-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b The stable shRAP80 #1 infected EC cells, EC109 (a) and EC1 (b), were treated with or without ATM inhibitor KU-55933 and then lysed to detect the indicated proteins using western blotting assays. The gray values were obtained from three independent experiments using Image J software and then subjected to ANOVA test. c EC1/shCon. or EC1/shRAP80 #1 cells pretreated with or without KU-55933 were starved in serum-free medium for 12 h and then recovered in fresh medium for 18 h, afterwards, these pellets were collected to analyze the cell cycle distribution using flow cytometry. The percentage of cells in G2/M phase from three independent experiments was represented as mean ± STD and statistically analyzed using the ANOVA test. d Pellets from above FACS analysis were lysed and subjected to examine G2/M checkpoint-specific proteins using western blotting assays. E. EC1/shCon. and EC1/shRAP80 #1 cells treated with or without ATM inhibitor KU-55933 were subjected to colony formation assays