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. 2018 Feb 2;9(2):146. doi: 10.1038/s41419-017-0177-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The whole-protein extracted from EC1/shCon. and EC1/shRAP80 #1 cells treated with 0.2 mg/ml CHX for indicated time points were subjected to western blot analysis to test the indicated proteins. The gray values were statistically analyzed. b The screening tests of E3 ligases and deubiquitylated enzymes in shCon. and shRAP80 #1 infected EC109 and EC1 cells using western blotting assays. c The whole-protein extracted from EC1/shCon. and EC1/shRAP80 #1 cells treated with 0.2 mg/ml CHX for indicated time points were subjected to western blot analysis to evaluate the stability of USP13. d, e The stable RAP80 knockdown EC109 and EC1 cells transfected with HA or HA-USP13 were subjected to colony formation analysis (d), the transfection efficiency was confirmed using western blotting assays (e)