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. 2018 Feb 14;9(2):240. doi: 10.1038/s41419-018-0296-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Prefer-fixed sections of wild-type a- d and rod-cre PKM2-KO e–h mouse retinas were subjected to immunofluorescence with anti-PKM2 a, e and PNA b, f. c, g Merged images of PKM2 and PNA. d, h Omission of primary antibody. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; ROS, rod outer segments; RIS, rod inner segment. Scale bar = 50 μm