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. 2018 Feb 14;9(2):227. doi: 10.1038/s41419-018-0307-5

Fig. 6. The role of AKT2 in FGF21-induced protection on cardiomyocytes against HG/Pal.

Fig. 6

Primary cardiomyocytes were isolated and treated with either control or AKT2-siRNA and then the cells were cotreated with both HG/Pal and FGF21 for 24 h. Western blotting was used to detect AKT phosphorylation (a, b) and AKT2 expression (c, d). The content of nuclear NRF2, Fyn (e, f), and cytosol NRF2, Fyn (g, h), and the expressions of 4-HNE (i), ANP (j), CTGF (k), and cleaved-caspase 3 (l) were also measured by western blot. Data were collected from at least three independent experiments and presented as mean ± SD. *P < 0.05 vs. control in the Con-siRNA group; #P < 0.05 vs. HG/Pal in the Con-siRNA group; @P < 0.05 vs. control in AKT2-siRNA group; &P < 0.05 vs. HG/Pal in the AKT2-siRNA group; $P < 0.05 vs. HG/Pal/FGF21 in the Con-siRNA group