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. 2018 Jan 26;9(2):127. doi: 10.1038/s41419-017-0149-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Total protein was extracted from normal cervix tissue, HPV-negative cervical cancer cell line C33A and two HPV16-positive cervical cancer cell lines SiHa and CaSki. Expression levels of E7 and p63 were assessed by western blotting. b Western blot analysis of E6 and E7 was carried out 48 h after transfection in CaSki cells to test the specificity and efficiency of the siRNAs. c and d p63 protein levels were measured 48 h following E7-depletion c or E6-depletion d in CaSki cells by western blotting. In all western blots GAPDH was used as equal loading control