Fig. 2. E7 promotes p63 expression at transcriptional level.

a CaSki cells were transfected with scrambled siRNA or E7-siRNA. Forty-eight hours later, cells were treated with the proteasome inhibitor MG132 (20 μM). The untreated control cells and MG132-treated cells were harvested 4 h later and expression of E7 and p63 were assessed by western blotting. The intensity of the bands was quantified by using the ImageJ software. b Q-PCR was used to quantitate E7 and p63 transcript levels in E7-depleted cells 48 h following transfection. c and d Total RNA from C33A, SiHa, and CaSki cells was extracted and reversed by using random primers. The cDNA was used to analyse the transcript levels of E7 (c) and p63 (d) by Q-PCR