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. 2018 Jan 24;7(1):7. doi: 10.1038/s41389-017-0022-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a EBAG9 silencing decreases EBAG9 protein expression in LNCaP cells. The cells were transfected with siRNA targeting EBAG9 (siEBAG9 #1) or non-targeting control siRNA (siControl). b siEBAG9 #1 inhibits LNCaP cell migration. Cells transfected with indicated siRNAs were seeded on the upper chamber and migrated cells were stained after 48 h. Representative images of migrated cells are shown. Scale bar, 20 μm. Migrated cell numbers were counted in 5 microscopic fields at least. Data are shown as mean ± SD (n = 5). c Effect of EBAG9 knockdown on EMT-related gene expression in LNCaP cells. qRT-PCR analyses for VIM, SNAI1, SNAI2, and EBAG9 mRNA were performed using RNAs prepared from LNCaP cells treated with siEBAG9 #1 or siControl. Data are shown as mean ± SD (n = 3). *P < 0.05; **P < 0.01 (two-sided Student’s t-test)