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. 2018 Jan 24;7(1):7. doi: 10.1038/s41389-017-0022-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a EBAG9 increases LNCaP cell migration. Method is described in Fig. 2b. Scale bar, 20 μm. The effect of EBAG9 overexpression on the migration of LNCaP cells is quantitated by counting the migrated cells in at least 5 fields under the microscope. The data are shown as mean ± SD (n = 5). b Regulation of EMT-related genes in LNCaP-EBAG9 cells. qRT-PCR analyses of VIM, SNAI1, SNAI2, and EBAG9 mRNA were performed using RNAs prepared from LNCaP-EBAG9 #4 and #6 and LNCaP-Vector #3 and #5 cells. Data are shown as mean ± SD (n = 3). *P < 0.05; **P < 0.01 (Statistical analysis was performed using the two-sided Mann–Whitney U-test)