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. 2018 Jan 24;7(1):7. doi: 10.1038/s41389-017-0022-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a EBAG9 protein is secreted in EVs. EVs were prepared from LNCaP-EBAG9 cells (EBAG9 #4 and #6) and control cells (Vector #3 and #5), and subjected to western blot analysis using EBAG9 antibody. b EVs are integrated into LNCaP cells. Cells were co-cultured with green fluorescent PKH67-labeled EVs for indicated times and co-stained with Cy3-labeled anti-vimentin antibody and 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 10 μm. c Cancer-derived EVs containing EBAG9 stimulate parental cell migration. LNCaP cells were incubated with LNCaP-EBAG9 cell-derived (EBAG9 #4 and #6) or control cell-derived (Vector #3 and #5) EVs, and cell migration was evaluated as described in Fig. 4. Data are shown as mean ± SD (n = 5). **P < 0.01 (Statistical analysis was performed using the two-sided Mann–Whitney U-test)