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. 2018 Feb 23;9(3):323. doi: 10.1038/s41419-018-0377-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig.1 — a Male B6 mice (6–10 weeks) were treated with necrotic or live HEK293 cells. Complete blood count were measured at different time points after necrotic cells (solid lines) or live cells (dashed lines) treatment. b Flow cytometry analysis of CD11b+Gr-1+ neutrophil percentage in PBMCs at different time points after necrotic cells treatment. c CD62L expression levels in CD11b+Gr-1+ neutrophil were compared at 0 h, 30 min, and 1 h after necrotic cells treatment. d Myeloperoxidase (MPO) staining in the liver from mice 1h after live or necrotic cells treatment. Arrows indicated MPO+ neutrophils. Magnification ×200. Data are expressed as mean ± SEM, n = 8 per group. *P < 0.05, **P < 0.05, ***P < 0.001