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. 2018 Feb 23;9(3):323. doi: 10.1038/s41419-018-0377-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig.3 — Male B6 mice (6–10 weeks) were treated with necrotic or live HEK293 cells. Control isotype antibody or CXCL1 blocking antibody (100 μg per mouse) was injected 15 min before necrotic cells treatment, total blood neutrophil number (a) and neutrophil percentage (b) were determined 1 h after necrotic cells treatment. c Mice were pre-treated with control or CXCL1 blocking antibody as a and b, liver samples were obtained and stained with MPO antibody 1 h after necrotic cells treatment. Arrows indicated MPO+ neutrophils. Magnification ×200. Data are expressed as mean ± SEM, n = 6 per group. **P < 0.01, ***P < 0.001