Fig.6. TNF-α but not IL-6 mediates the production of CXCL1 in hepatocytes.

Male B6 mice (6~10 weeks) were treated with necrotic cells. a Serum levels of proinflammatory cytokines such as IL-6, IFN-γ, TNF-α, IL-12, IL-1β were measured 0, 30 min, and 1 h after necrotic cells treatment. b, c Male B6 mice (6–10 weeks) were pre-treated with clodronate liposomes or control liposomes, 24 h later mice received necrotic cells. Serum IL-6 and TNF-α levels were measured 1 h after cell treatment. d–g Control isotype antibody or IL-6, TNF-α blocking antibody (100 ng per mouse) was injected 15 min before necrotic cells treatment, CXCL1 concentration (d, f) and neutrophil number (e, g) were determined 1 h after necrotic cells treatment. Data are expressed as mean ± SEM, n = 6 per group. **P < 0.01, ***P < 0.001