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. 2018 Feb 7;9(2):196. doi: 10.1038/s41419-017-0239-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a B4GalT5 expression in subcutaneous adipose tissue from subjects with neither obesity nor diabetes (n = 12) vs. those without obesity but with diabetes (n = 9); and from subjects with obesity but without diabetes (n = 6) vs. with both obesity and diabetes (n = 13). b Correlation analysis between BMI and B4GalT5 mRNA levels in subcutaneous adipose tissue of human (n = 52). c–d qPCR analysis of B4GalT5 mRNA expression and western blotting detect B4GalT5 protein level in inguinal (subcutaneous) adipose tissue of C57BL/6 J mice fed with ND or HFD for 12 weeks (n = 14), and ob/ob mice (n = 8) and their wild-type(WT) littermates (n = 8). e B4GalT5 mRNA expression in stromal vascular fraction (SVF) or mature adipocytes fraction (AD) isolated from inguinal adipose tissue of C57BL/6 J mice fed with HFD for 12 weeks (n = 6). f The relative mean fluorescence intensity (MFI) by flow cytometric analysis of SVF and AD for B4GalT5 expression in inguinal adipose tissue of C57BL/6 J mice fed with HFD for 12 weeks (n = 6). Statistical analysis was performed by collating the results from multiple samples. *P < 0.05, **P < 0.01, ***P < 0.001