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. 2018 Feb 7;9(2):196. doi: 10.1038/s41419-017-0239-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — BMDMs from 6-week-old C57BL/6 J mice were treated with 100 ng/ml LPS or 10ng/ml IL-4 or vehicle control for 24 h. B4GalT5 was silenced by siB4GalT5 in BMDMs, downregulation of B4GalT5 by shRNA in RAW264.7 macrophages. a qPCR analysis of the mRNA level of B4GalT5 on different groups. b Flow cytometry analysis of the expression of F4/80, CD11c, and CD206. Percentages of F4/80⁺CD11c⁺ cells and F4/80⁺CD206⁺ cells are shown. c Relative mRNA abundance of M1 macrophage marker genes. d Relative mRNA abundance of M2 macrophage marker genes. e Relative mRNA abundance of M1 macrophage marker genes. f Relative mRNA abundance of M2 macrophage marker genes. g Western blotting was conducted to measure the level of the indicated protein. Each data is representative of at least three independent experiments with identical results. *P < 0.05, **P < 0.01, ***P < 0.001