Fig. 2. Regulating the expression of let-7c can affect self-renewal of cholangiocarcinoma cells in vitro.

a Sphere formation with upregulated let-7c transduced TFK-1 cells at multiple time points. b Shpere formation with downregulated let-7c transduced HUCCT-1 cells at multiple time points. c, d Quantification of cell numbers per sphere. (e and f) The number of spheres per 1000 cells indicates the capacity for sphere formation. The data were compared with the negative control group. *P < 0.05, **P < 0.01. NC negative control group; 7C+ let-7c-upregulated group; 7c− let-7c-downregulated group