Fig. 6. Let-7c directly targets EZH2 and indirectly affects β-catenin via DVL3.

a The heat map of mRNA array showed the differential gene expression upon let-7c inhibition in HUCCT-1 cells. b, c The expression of DVL3, β-catenin, E-cadherin, N-cadherin, and Vimentin. β-actin was used as the loading control. All data were compared with the NC group. d The expression of β-catenin and EZH2 in paired cholangiocarcinoma and paratumor tissues from cholangiocarcinoma patients. e The quantification of western blot results. **P < 0.01; N Normal bile duct tissue; T cholangiocarcinoma (tumor group), BC blank control group; NC negative control group; 7C+ let-7c-upregulated group; 7c− let-7c-downregulated group