Fig. 7. EZH2 and DVL3/β-catenin axis potentially participate in let-7c regulating the malignant biological behavior of cholangiocarcinoma.

Luciferase reporter assay for (a) EZH2, (b) DVL3 and (c) β-catenin. d The effect of transfection with siEZH2 and let-7c downregulated plus siEZH2 on the invasive capacity of TFK-1 cells. e Weights per mouse of distant metastasis model. f The number of spheres per 1000 cells indicates the ability of shEZH2 suppressing capacity of sphere formation. g BALB/c nude mice weights and (h) tumor diameter of subcutaneous injected mice model. **P < 0.01. WT wild type; Mut mutant type; NC negative control group; 7C+ let-7c-upregulated group; 7C-I let-7c-downregulated group; siEZH2 Small Interfering EZH2; shEZH2 Small hairpin EZH2; shDVL3 Small hairpin DVL3