Fig. 3. ANXA3 knockdown inhibits in vivo tumor metastasis but promotes tumor growth.

a For each group, 50,000 MDA-MB-231 cells were implanted into the mammary glands of 6- to 8-week-old female nude mice and tumor size was monitored weekly. **p < 0.01; ***p < 0.001. b The tumor images (left) and tumor weight (right) from a are shown. The tumor growth from a was halted when the largest tumor reached 15 mm in diameter for humane reasons. **p < 0.01; ***p < 0.001. c IHC staining of ANXA3 and Ki67 in the tumors from b. d Quantification of Ki67-positive cells (%) as shown in c. **p < 0.01; ***p < 0.001. e HE staining in slices of lungs harvested from mice in a. Arrow shows the metastatic nodules. f Quantitative analysis of the metastatic nodules in e. *p < 0.05