Fig. 5. ANXA3-knockdown induces MET and affects breast cancer cell invasion and proliferation via inhibiting the NFκB pathway.

a Heatmap of gene expression in shCTRL- and shANXA3-infected MDA-MB-231 cells; each group has been repeated twice. b Pathway enrichment analysis of transcriptome profiling results from a. c Diagram showing the fold-changes of both the transcriptome sequencing and RT-PCR experiments of several typical genes to confirm and validate the sequencing data. d The protein levels of NFKBIA (IκBα) and phospho-p65 (p-p65) were detected by western blotting. e The protein levels of ANXA3, IκBα, phospho-p65, E-cadherin, and Vimentin were detected by western blotting. f Cell invasion ability was measured by the Matrigel invasion assay, as described in the methods. g Quantitative analysis of the total number of invaded cells in f. **p < 0.01. h Cell proliferation activity was measured by an MTT assay as described in the methods. *p < 0.05; ***p < 0.001. i Flow cytometry analyses of BCSCs by the ALDEFLUOR assay (upper) and the CD24/CD44 assay (lower) in shCTRL-, shANXA3-, shIκBα-, and shANXA3 + shIκBα-infected MDA-MB-231 cells. *p < 0.05; ***p < 0.001