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. 2018 Feb 19;9(3):291. doi: 10.1038/s41419-018-0317-3

Fig. 6. ALLN inhibits the activity of CAPN7.

Fig. 6

a Ishikawa cells were transfected with ITGB3-Luc and were treated with ALLM, ALLN, and PD150606 (10 μM). After 48 h, the luciferase activities were measured and are presented as fold induction. Values represent the mean ± SEM (n = 3). ***p < 0.001. b Ishikawa cells were transfected with ITGB3-Luc and Myc-HOXA10, and were treated with ALLM, ALLN, and PD150606 (10 μM). After 48 h, the luciferase activities were measured and are presented as fold induction. Values represent the mean ± SEM (n = 3). *p < 0.05, **p < 0.01. c Western blot analysis of whole-cell lysates from Ishikawa cells treated with ALLM, ALLN, and PD150606 (10 μM), as indicated. The membrane was probed with the indicated antibodies. d Calpain inhibitor ALLN inhibited the degradation of HOXA10 by CAPN7. e The intensities of HOXA10 signals were quantified from three independent experiments and normalized to the control group. *p < 0.05, **p < 0.01. f ALLN inhibited CAPN7-induced downregulation of transcriptional activity. Values represent the mean ± SEM (n = 3); *p < 0.05, **p < 0.01. g ALLN reversed GFP-CAPN7-downregulated HOXA10 transcriptional activity. Values represent the mean ± SEM (n = 3); *p < 0.05, **p < 0.01. h ALLN suppressed CAPN7-induced BeWo adhesion. Values represent the mean ± SEM (n = 3); **p < 0.01. i ALLN rescued the CAPN7-downregulated HOXA10 DNA-binding ability