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. 2018 Jan 26;9(2):136. doi: 10.1038/s41419-017-0167-4

Fig. 2. SIRT1 promotes the EMT and increase metastatic potential in melanoma cells.

Fig. 2

a Sk-Mel-28 cells stably expressing vector or Flag-SIRT1 were harvested and protein expression was detected by western blot analysis. b, c Sk-Mel-28 cells stably expressing vector or Flag-SIRT1 were plated in the upper chamber of the transwell filters for 24 h. Cells migrating to the underside of the transwell insert were then counted. d A375-shSIRT1 and control cells were harvested, and protein expression was detected by western blot analysis. e, f A375-shSIRT1 and control cells were plated in the upper chamber of the transwell filters for 24 h. Cells migrating to the underside of the transwell insert were then counted. g Immunofluorescence staining of actin filaments (Phalloidin) in Sk-Mel-28 cells stably expressing either vector or Flag-SIRT1. Bar = 10 μm