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. 2018 Jan 26;9(2):136. doi: 10.1038/s41419-017-0167-4

Fig. 5. SIRT1 promotes cell migration and invasion by autophagic degradation of E-cadherin.

Fig. 5

a, b Sk-Mel-28 cells stably expressing vector or Flag-SIRT1 were plated in the upper chamber of transwell filters for 24 h and then treated with PBS or CQ (100 mM, 12 h). Cells migrating to the underside of the transwell insert were subsequently counted. c Sk-Mel-28 cells stably expressing vector or Flag-SIRT1 were treated with PBS or CQ (100 mM, 12 h), and protein expression was measured by western blotting. d, e Sk-Mel-28 cells stably expressing vector or Flag-SIRT1 were transfected with HA-tagged Beclin 1 (WT, 2KR) for 24 h. Cells were plated in the upper chamber of transwell filters for 24 h, and then cells migrating to the underside of the transwell insert were counted. f Sk-Mel-28 cells stably expressing vector or Flag-SIRT1 were transfected with HA-tagged Beclin 1 (WT, 2KR) for 24 h, and protein expression was then measured by western blotting