Fig. 2. Chemoresistance of ALCAM⁺ GCTB cells.

a Cell viability of GCTB28 cells after 48 h treatment with cisplatin(DDP) at indicated concentrations by the CCK8 assay(left panel). Light microscopy showed floating dead GCTB28 cells 7 days after chemotherapy with DDP. Surviving cells adhered at the bottom of the culture dish(right panel). (Scale bar = 20 µm). b Flow cytometry of ALCAM in GCTB28 cells 7 days after chemotherapy. Independent experiments were repeated three times. Data were presented as mean ± s.d. (***p < 0.001; **p < 0.01; *p < 0.05). c and d) Flow cytometry for apoptosis in ALCAM⁺ and ALCAM⁻ cells from GCTB28 cells (c) and clinical samples (cases #34 and #39) (d) after chemotherapy with DDP