Fig. 8. Autophagy is defective in renal proximal tubules of PT-Atg7 KO mice.

Wild-type (PT-Atg7 WT) and PT-Atg7 KO mice were injected with saline or a single dose of cisplatin (30 mg/kg, i.p.) in the absence (−) or presence (+) of TSA (1 mg/kg, i.p., daily injection) (n = 5–6 for each condition). Kidneys were collected 3 days after treatment for immunoblot analysis and immunohistochemical staining of LC3B. a Representative immunoblots. β-Actin was loading control. b Densitometric analysis of LC3B signals. c Representative images of LC3B immunohistochemical staining (×400). Scale bar: 15 μm. d Quantitative analysis of punctate LC3B staining. Data in b and d are expressed as mean ± SD. *P < 0.05, significantly different from the saline group. ^#P < 0.05, significantly different from the cisplatin-only group. ^P < 0.05, significantly different from the corresponding groups of PT-Atg7 WT mice