Figure 4.

KLT induces apoptosis in hepatocellular carcinoma cells.

Notes: (A) Cells were stained with Hoechst 33342 (5 μg/mL) and subjected to analysis of apoptosis population (n=3). (B) PE-Annexin V staining of phosphatidylserine exposed on the cell surface was measured by flow cytometric analysis (n=3). Data derived from three separate experiments are presented as the mean ± SD. *P<0.05 and **P<0.01 vs control (one-way ANOVA, post hoc comparisons, and Tukey’s test). Columns represent the mean from three independent experiments, and bars represent SDs.

Abbreviations: ANOVA, analysis of variance; KLT, Kanglaite; PI, propidium iodide.