Fig. 5. Recombinant protein N4-T attenuates p-eIF2α in the presence of γ34.5.
a HeLa cells were exposed to HSV-1/F at 0.1 MOI for indicated times, in the presence of PBS or N4-T of 20 μg/ml. Viral yields were determined by plaque assay and were presented as log10 PFU/ml. Values represent means of triplicates with SD. *p ≤ 0.05; **p ≤ 0.01. b HeLa cells were infected with HSV-1/F at 0.01 MOI in the presence of PBS or N4-T of 20 μg/ml. Expression of HSV-ICP8 was detected by Western blotting at 24, 36, or 48 h.p.i., and actin served as the loading control. c-e HeLa cells infected with HSV-1/F c, HSV-1/Δγ34.5 d, or NDV e were treated with N4-T at different concentrations of 0, 10, or 20 μg/ml. Cell lysates prepared 12 h.p.i. were then analyzed by Western blotting with the indicated antibodies. Shown is one representative of two independent experiments